Hotspot

accD

LSC · 60961-62037 · 1077 bp

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Pi 0.074

MarkerSeek score 83.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1077
variable_sites: 232
pi: 0.074
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CGGCTGCGGAAAAAGAATTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.9	58.0	1578 bp	0.076923	42.3
accD_p2	CGGCTGCGGAAAAAGAATTT	ATACGTTCTGATCGCCAACC	45.0	50.0	57.9	58.1	1843 bp	0.076923	42.0
accD_p3	CGGCTGCGGAAAAAGAATTT	AGATTTGTCTCTACTGCGCG	45.0	50.0	57.9	58.1	1642 bp	0.076923	41.9
accD_p4	CGGCTGCGGAAAAAGAATTT	AAGGAAGCCATTGCAATTGC	45.0	45.0	57.9	57.9	1585 bp	0.076923	41.7
accD_p5	AGAAATGGTAGTTCGGCTGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1591 bp	0.076923	40.8