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clpP-psbB

LSC · 74266-74811 · 546 bp

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Pi 0.0691

MarkerSeek score 81.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 117
pi: 0.0691
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CAGAGCCTGGATGCTTCATT	ACACCCATATTCCAGGACCA	50.0	50.0	57.9	58.0	1342-1508 bp	1.0	91.4
clpP-psbB_p2	CAGAGCCTGGATGCTTCATT	AATGCGCCAAAACCAAAACA	50.0	40.0	57.9	58.0	1304-1470 bp	1.0	91.3
clpP-psbB_p3	CAGAGCCTGGATGCTTCATT	CAGATAGCTGCCAAGAAGCA	50.0	50.0	57.9	58.0	1175-1341 bp	1.0	91.3
clpP-psbB_p4	CAGAGCCTGGATGCTTCATT	AGATAGCTGCCAAGAAGCAC	50.0	50.0	57.9	58.0	1174-1340 bp	1.0	91.3
clpP-psbB_p5	CAGAGCCTGGATGCTTCATT	TTTGTACAGACGTTGGGGTG	50.0	50.0	57.9	58.0	1518-1684 bp	1.0	91.2