Hotspot

ycf4

LSC · 57646-58203 · 558 bp

Back to Phalaris

Pi 0

MarkerSeek score 24.30

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 558
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TTTCCGGCAATTGCAATGAC	CCGCTTCATTCGATGACTCT	45.0	50.0	57.9	57.8	1168-1283 bp	1.0	78.4
ycf4_p2	TTTCCGGCAATTGCAATGAC	CCCCAGGGCAAAAATACGAT	45.0	50.0	57.9	58.2	1265-1380 bp	1.0	78.3
ycf4_p3	TTTCCGGCAATTGCAATGAC	ATGAACCCGCTTCATTCGAT	45.0	45.0	57.9	57.7	1174-1289 bp	1.0	77.5
ycf4_p4	TTTCCGGCAATTGCAATGAC	GAACCCGCTTCATTCGATGA	45.0	50.0	57.9	58.3	1172-1287 bp	1.0	77.4
ycf4_p5	TTTCCGGCAATTGCAATGAC	TTCGGATTGCCTGGTATTCC	45.0	50.0	57.9	57.7	1335-1450 bp	1.0	77.3