Hotspot

rpoA

LSC · 73609-74628 · 1020 bp

Back to Phalaris

Pi 0.00882

MarkerSeek score 63.80

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 9
pi: 0.00882
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	ATGATCCATTTCGCGAAAAT	ACACCTATGCCGCATAATGG	35.0	50.0	54.0	58.1	1242-1243 bp	1.0	48.0
rpoA_p2	ATGATCCATTTCGCGAAAAT	CACCTATGCCGCATAATGGA	35.0	50.0	54.0	57.8	1241-1242 bp	1.0	47.3
rpoA_p3	ATGATCCATTTCGCGAAAAT	GGATGTCGACCGCCTAAAAA	35.0	50.0	54.0	58.3	1224-1225 bp	1.0	46.7
rpoA_p4	ATGATCCATTTCGCGAAAAT	TAATGGATGTCGACCGCCTA	35.0	50.0	54.0	58.3	1228-1229 bp	1.0	46.4
rpoA_p5	ATGATCCATTTCGCGAAAAT	ATGTAACACCTATGCCGCAT	35.0	45.0	54.0	57.4	1247-1248 bp	1.0	43.7