Hotspot

rpl32-trnL-UAG

SSC · 104770-105428 · 659 bp

Back to Phalaris

Pi 0.0228

MarkerSeek score 57.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 659
variable_sites: 15
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTTTTTCGGGGCAACAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.0	58.0	760 bp	1.0	81.6
rpl32-trnL-UAG_p2	GTTTTTCGGGGCAACAACAA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.0	58.0	1312 bp	1.0	80.7
rpl32-trnL-UAG_p3	TTTTTCGGGGCAACAACAAC	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.0	58.0	759 bp	0.5	59.9
rpl32-trnL-UAG_p4	TTTTTCGGGGCAACAACAAC	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.0	58.0	1311 bp	0.5	59.7
rpl32-trnL-UAG_p5	TTTTTCGGGGCAACAACAAC	GGATTCGAACCGAGATGCTT	45.0	50.0	58.0	58.1	762 bp	0.5	59.4