Hotspot

rpl20-rps12

LSC · 66015-66712 · 698 bp

Back to Phalaris

Pi 0.0215

MarkerSeek score 65.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 15
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	45.0	55.0	58.0	58.0	774-775 bp	1.0	81.3
rpl20-rps12_p2	ACGCATTTTTGTTCGTCGTC	CAACATGCCGACGGTTAAAC	45.0	50.0	58.0	58.0	869-870 bp	1.0	81.0
rpl20-rps12_p3	ACGCATTTTTGTTCGTCGTC	CATGCCGACGGTTAAACAAC	45.0	50.0	58.0	58.0	866-867 bp	1.0	81.0
rpl20-rps12_p4	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	45.0	55.0	58.0	58.1	777-778 bp	1.0	81.0
rpl20-rps12_p5	ACGCATTTTTGTTCGTCGTC	ATGCCGACGGTTAAACAACT	45.0	45.0	58.0	58.1	865-866 bp	1.0	80.5