Hotspot

petG-trnW-CCA

LSC · 63309-63430 · 122 bp

Back to Phalaris

Pi 0.0738

MarkerSeek score 80.00

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 9
pi: 0.0738
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CGGGATTATTCGTGACTGCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	256 bp	1.0	82.2
petG-trnW-CCA_p2	CGGGATTATTCGTGACTGCA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	220 bp	1.0	82.0
petG-trnW-CCA_p3	AGCGGGATTATTCGTGACTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	258 bp	1.0	80.9
petG-trnW-CCA_p4	AGCGGGATTATTCGTGACTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	222 bp	1.0	80.7
petG-trnW-CCA_p5	CGGGATTATTCGTGACTGCA	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	58.1	57.7	254 bp	1.0	79.5