Hotspot

petL-petG

LSC · 63024-63194 · 171 bp

Back to Phalaris

Pi 0.0117

MarkerSeek score 52.90

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 2
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	ACCCCAGCTCTATTTATTGGC	TGCAGTCACGAATAATCCCG	47.6	50.0	57.8	58.1	291 bp	1.0	70.2
petL-petG_p2	ACCCCAGCTCTATTTATTGGC	GATCTAACTGATCCCCACGC	47.6	55.0	57.8	57.8	325 bp	1.0	69.4
petL-petG_p3	ACCCCAGCTCTATTTATTGGC	CAGTCACGAATAATCCCGCT	47.6	50.0	57.8	57.8	289 bp	1.0	68.9
petL-petG_p4	CCCCAGCTCTATTTATTGGCT	TGCAGTCACGAATAATCCCG	47.6	50.0	57.5	58.1	290 bp	1.0	67.9
petL-petG_p5	CCCCAGCTCTATTTATTGGCT	GATCTAACTGATCCCCACGC	47.6	55.0	57.5	57.8	324 bp	1.0	67.1