Hotspot

psbE-petL

LSC · 61785-62927 · 1143 bp

Back to Phalaris

Pi 0.00955

MarkerSeek score 65.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 10
pi: 0.00955
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GCCAATAAATAGAGCTGGGGT	45.0	47.6	58.0	57.8	1237-1330 bp	1.0	74.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCAATAAATAGAGCTGGGGT	50.0	47.6	57.9	57.8	1226-1319 bp	1.0	74.4
psbE-petL_p3	ACCAACCCGCAATGAATAGG	GCCAATAAATAGAGCTGGGGT	50.0	47.6	58.2	57.8	1228-1321 bp	1.0	72.9
psbE-petL_p4	TGACAAATAACCAACCCGCA	AGCCAATAAATAGAGCTGGGG	45.0	47.6	58.0	57.5	1238-1331 bp	1.0	72.4
psbE-petL_p5	CAACCCGCAATGAATAGGGA	AGCCAATAAATAGAGCTGGGG	50.0	47.6	57.9	57.5	1227-1320 bp	1.0	72.1