Hotspot

rbcL-psaI

LSC · 55613-57194 · 1582 bp

Back to Phalaris

Pi 0.0127

MarkerSeek score 54.00

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1582
variable_sites: 20
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GAGCCGGTAGATACTATCGA	GTCATTGCAATTGCCGGAAA	50.0	45.0	55.5	57.9	1686-1722 bp	1.0	60.6
rbcL-psaI_p2	GAGCCGGTAGATACTATCGA	GAAGTCATTGCAATTGCCGG	50.0	50.0	55.5	58.4	1689-1725 bp	1.0	58.3
rbcL-psaI_p3	GAGCCGGTAGATACTATCGAT	GTCATTGCAATTGCCGGAAA	47.6	45.0	56.0	57.9	1686-1722 bp	1.0	57.2
rbcL-psaI_p4	GAGCCGGTAGATACTATCGA	AGAAGTCATTGCAATTGCCG	50.0	45.0	55.5	57.4	1690-1726 bp	1.0	56.1
rbcL-psaI_p5	GAGCCGGTAGATACTATCGA	AGTCATTGCAATTGCCGGAA	50.0	45.0	55.5	58.7	1687-1723 bp	1.0	55.3