Hotspot

trnT-UGU-trnL

LSC · 45496-46301 · 806 bp

Back to Phalaris

Pi 0.0125
MarkerSeek score 64.50
Primer available yes
Label trnT-UGU-trnL

Feature Metadata

label_name
trnT-UGU-trnL
feature_type
igs
region
LSC
length_bp
806
variable_sites
10
pi
0.0125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 910-913 bp 1.0 80.9
trnT-UGU-trnL_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 902-905 bp 1.0 80.6
trnT-UGU-trnL_p3 ATTTGAACCGATGACCCTCG CGATTTCGCCATATCCCCAT 50.0 50.0 58.0 57.9 887-890 bp 1.0 80.1
trnT-UGU-trnL_p4 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 880-883 bp 1.0 80.1
trnT-UGU-trnL_p5 CGATGACCCTCGCATTACAA CGATTTCGCCATATCCCCAT 50.0 50.0 58.1 57.9 879-882 bp 1.0 79.7