Hotspot

trnR-UCU-rps14

LSC · 35627-35980 · 354 bp

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Pi 0.0169

MarkerSeek score 63.40

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 6
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	TATCCGGACACGTACTTCGA	47.6	50.0	56.9	58.0	461 bp	1.0	64.4
trnR-UCU-rps14_p2	AGGTTTAGAAGACCTCTGTCCT	TATCCGGACACGTACTTCGA	45.5	50.0	57.9	58.0	467 bp	1.0	63.8
trnR-UCU-rps14_p3	AGAAGACCTCTGTCCTATCCA	GCTATCCGGACACGTACTTC	47.6	55.0	56.9	57.9	463 bp	1.0	63.7
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	420 bp	1.0	63.3
trnR-UCU-rps14_p5	AGGTTTAGAAGACCTCTGTCCT	GCTATCCGGACACGTACTTC	45.5	55.0	57.9	57.9	469 bp	1.0	63.0