Hotspot

rps16-trnQ-UUG

LSC · 5561-6343 · 783 bp

Back to Phalaris

Pi 0.00786

MarkerSeek score 65.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 6
pi: 0.00786
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	836-856 bp	1.0	80.7
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	847-867 bp	1.0	80.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	45.0	45.0	58.1	57.8	905-925 bp	1.0	79.8
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	840-860 bp	1.0	79.6
rps16-trnQ-UUG_p5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	50.0	45.0	58.2	57.8	916-936 bp	1.0	79.2