Hotspot

ndhD

SSC · 114229-115317 · 1089 bp

Back to Phalaenopsis

Pi 0.0304

MarkerSeek score 70.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1089
variable_sites: 100
pi: 0.0304
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTGGAGCAGTATGGGCTAA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	1377-1911 bp	1.0	89.6
ndhD_p2	TTCTGGAGCAGTATGGGCTA	AGATGTGAATCCGCTTGTCC	50.0	50.0	57.8	58.0	1378-1912 bp	1.0	89.6
ndhD_p3	TCTGGAGCAGTATGGGCTAA	GTACCCAATGTGTTCGAGCT	50.0	50.0	57.8	57.9	1493-2027 bp	1.0	88.6
ndhD_p4	TCTGGAGCAGTATGGGCTAA	GCACGGGCTTTTCTGGTATA	50.0	50.0	57.8	58.0	1221-1728 bp	0.642857	73.0
ndhD_p5	TTCTGGAGCAGTATGGGCTA	GCACGGGCTTTTCTGGTATA	50.0	50.0	57.8	58.0	1222-1729 bp	0.642857	73.0