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PhapfoPp003

LSC · 1428-4395 · 2968 bp

Back to Phalaenopsis

Pi 0.0233

MarkerSeek score 62.20

Primer available yes

Label PhapfoPp003

Feature Metadata

label_name: PhapfoPp003
feature_type: gene
region: LSC
length_bp: 2968
variable_sites: 198
pi: 0.0233
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
PhapfoPp003_p1	CAGAACGGCGTATGTGTGTA	TCTTAGTGCCTGATGTGGGA	50.0	50.0	58.0	58.0	693-718 bp	0.428571	65.8
PhapfoPp003_p2	CAGAACGGCGTATGTGTGTA	AGTGCCTGATGTGGGAAAAG	50.0	50.0	58.0	58.1	689-714 bp	0.428571	65.5
PhapfoPp003_p3	CAGAACGGCGTATGTGTGTA	GCAACGAGCTTCCCTTCTTA	50.0	50.0	58.0	57.9	759-784 bp	0.428571	65.2
PhapfoPp003_p4	CAGAACGGCGTATGTGTGTA	CCTGATGTGGGAAAAGGGTC	50.0	55.0	58.0	58.2	685-710 bp	0.428571	64.9
PhapfoPp003_p5	CAGAACGGCGTATGTGTGTA	ATAAGACCGCGACTGATCCT	50.0	50.0	58.0	58.3	958-1020 bp	0.428571	63.2