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clpP-psbB

LSC · 73318-73939 · 622 bp

Back to Phalaenopsis

Pi 0.0408

MarkerSeek score 74.30

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 61
pi: 0.0408
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	AGGCACTTTTGGAACACCAA	CGATACCAAGGCAAACCCAT	45.0	50.0	58.1	58.2	602-741 bp	1.0	88.2
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	597-736 bp	1.0	88.0
clpP-psbB_p3	TTTGGAACACCAATGGGCAT	CGATACCAAGGCAAACCCAT	45.0	50.0	58.3	58.2	595-734 bp	1.0	87.2
clpP-psbB_p4	TCCTCTCCAGGATTCCGAAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	622-761 bp	1.0	87.1
clpP-psbB_p5	AGGCACTTTTGGAACACCAA	ACACGATACCAAGGCAAACC	45.0	50.0	58.1	58.5	605-744 bp	1.0	86.3