Pi
0.0381
MarkerSeek score
75.70
Primer available
yes
Label
PhapfoPt014-PhapfoPt015
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| PhapfoPt014-PhapfoPt015_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 651-704 bp | 1.0 | 90.9 |
| PhapfoPt014-PhapfoPt015_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 659-712 bp | 1.0 | 90.4 |
| PhapfoPt014-PhapfoPt015_p3 | GCGATGCTCTAACCTCTGAG | ATCCAATCAAGTCCGTAGCG | 55.0 | 50.0 | 57.9 | 57.8 | 674-727 bp | 1.0 | 89.1 |
| PhapfoPt014-PhapfoPt015_p4 | GCGATGCTCTAACCTCTGAG | AGTCCGTAGCGTCTACCAAT | 55.0 | 50.0 | 57.9 | 58.2 | 665-718 bp | 1.0 | 89.0 |
| PhapfoPt014-PhapfoPt015_p5 | GCGATGCTCTAACCTCTGAG | CAATCAAGTCCGTAGCGTCT | 55.0 | 50.0 | 57.9 | 57.7 | 671-724 bp | 1.0 | 88.7 |