Hotspot

rps16-PhapfoPt001

LSC · 6083-6947 · 865 bp

Back to Phalaenopsis

Pi 0.0359

MarkerSeek score 74.70

Primer available yes

Label rps16-PhapfoPt001

Feature Metadata

label_name: rps16-PhapfoPt001
feature_type: igs
region: LSC
length_bp: 865
variable_sites: 91
pi: 0.0359
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-PhapfoPt001_p1	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	849-994 bp	1.0	89.6
rps16-PhapfoPt001_p2	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	853-998 bp	1.0	88.5
rps16-PhapfoPt001_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	838-983 bp	0.928571	87.1
rps16-PhapfoPt001_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	842-987 bp	0.928571	86.0
rps16-PhapfoPt001_p5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	45.0	50.0	58.1	57.6	844-989 bp	0.928571	84.2