Hotspot

matK-rps16

LSC · 3256-4883 · 1628 bp

Back to Phalaenopsis

Pi 0.0336

MarkerSeek score 74.10

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1628
variable_sites: 165
pi: 0.0336
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	TCTTGTGCAGCGAAAATCCA	TCGATCCCGAAGAGAAGGAA	45.0	50.0	58.4	57.9	2094-2185 bp	1.0	86.8
matK-rps16_p2	TCTTGTGCAGCGAAAATCCA	GCTCAACCCACAGAAACTGT	45.0	50.0	58.4	58.3	1985-2076 bp	1.0	85.6
matK-rps16_p3	TCTTGTGCAGCGAAAATCCA	TGCTCAACCCACAGAAACTG	45.0	50.0	58.4	58.3	1986-2077 bp	1.0	85.6
matK-rps16_p4	CTTGTGCAGCGAAAATCCAT	TCGATCCCGAAGAGAAGGAA	45.0	50.0	57.4	57.9	2093-2184 bp	1.0	84.8
matK-rps16_p5	TCTTGTGCAGCGAAAATCCA	GTGCTCAACCCACAGAAACT	45.0	50.0	58.4	58.3	2000-2076 bp	0.642857	68.1