Hotspot

ndhA

SSC · 122957-125170 · 2214 bp

Pi 0.0034

MarkerSeek score 52.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 16
pi: 0.0034
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTCTTTCTCTTGGTTTAGA	TTGCAAATTCTTCCCCAGCT	31.8	45.0	53.2	58.0	2397-2399 bp	1.0	40.6
ndhA_p2	TGTTTCTTTCTCTTGGTTTAGA	GTCCACCCGGTTTCATCAAT	31.8	50.0	53.2	58.2	2417-2419 bp	1.0	40.6
ndhA_p3	ATGTTTCTTTCTCTTGGTTTAGA	TTGCAAATTCTTCCCCAGCT	30.4	45.0	53.9	58.0	2398-2400 bp	1.0	40.6
ndhA_p4	TGTTTCTTTCTCTTGGTTTAGAT	TTGCAAATTCTTCCCCAGCT	30.4	45.0	53.9	58.0	2397-2399 bp	1.0	40.6
ndhA_p5	TGTTTCTTTCTCTTGGTTTAGAT	GTCCACCCGGTTTCATCAAT	30.4	50.0	53.9	58.2	2417-2419 bp	1.0	40.6