Hotspot

rps8-rpl14

LSC · 83254-83491 · 238 bp

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Pi 0.0249
MarkerSeek score 51.50
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
238
variable_sites
11
pi
0.0249
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 GGTCGGCTGATACGCTTTAA AATCAAAGAAGCAGTGCCCA 50.0 45.0 58.1 58.0 721-730 bp 1.0 82.5
rps8-rpl14_p2 GGTCGGCTGATACGCTTTAA ATCAAAGAAGCAGTGCCCAA 50.0 45.0 58.1 58.0 720-729 bp 1.0 82.5
rps8-rpl14_p3 ATAAAACTGCGCCGGATTCT AATCAAAGAAGCAGTGCCCA 45.0 45.0 58.0 58.0 1115-1124 bp 1.0 81.9
rps8-rpl14_p4 ATAAAACTGCGCCGGATTCT ATCAAAGAAGCAGTGCCCAA 45.0 45.0 58.0 58.0 1114-1123 bp 1.0 81.9
rps8-rpl14_p5 ATAAAACTGCGCCGGATTCT GTTAAACCGGGCCGAATACT 45.0 50.0 58.0 58.0 1492-1501 bp 1.0 81.1