Hotspot

psbB-psbT

LSC · 76122-76657 · 536 bp

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Pi 0.0147
MarkerSeek score 52.60
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
536
variable_sites
17
pi
0.0147
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 TCAGAGATGTTTTTGCCGGT GGAATGTATAAACCAATGCTTCCA 45.0 37.5 57.7 57.5 665-705 bp 1.0 45.4
psbB-psbT_p2 GCTCAAGTGGAATTTGGAGC GGAATGTATAAACCAATGCTTCCA 50.0 37.5 57.4 57.5 627-667 bp 1.0 43.5
psbB-psbT_p3 TCAGAGATGTTTTTGCCGGT AGGAATGTATAAACCAATGCTTCC 45.0 37.5 57.7 57.3 666-706 bp 1.0 43.4
psbB-psbT_p4 GCCAGAACCTTGTTCAGAGA GGAATGTATAAACCAATGCTTCCA 50.0 37.5 57.4 57.5 678-718 bp 1.0 43.2
psbB-psbT_p5 GCCAGAACCTTGTTCAGAGA AGGAATGTATAAACCAATGCTTCC 50.0 37.5 57.4 57.3 679-719 bp 1.0 43.2