Hotspot

clpP-psbB

LSC · 73834-74594 · 761 bp

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Pi 0.023
MarkerSeek score 71.00
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
761
variable_sites
33
pi
0.023
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.92
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 881-939 bp 1.0 86.0
clpP-psbB_p2 TATACGTCAACCCAAGCTGC GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 876-934 bp 1.0 85.8
clpP-psbB_p3 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 799-857 bp 1.0 85.3
clpP-psbB_p4 TATACGTCAACCCAAGCTGC CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 794-852 bp 1.0 85.2
clpP-psbB_p5 CTTTTGGAACACCAATGGGC GGCCCAACCAGAAACTAGAG 50.0 55.0 57.8 57.9 827-885 bp 1.0 84.8