Hotspot

psbE-petL

LSC · 66173-67380 · 1208 bp

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Pi 0.00708
MarkerSeek score 55.60
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1208
variable_sites
16
pi
0.00708
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GCTGGGGTTATAGTTGAAGCA 50.0 47.6 57.9 58.0 995-1371 bp 1.0 76.6
psbE-petL_p2 CAACCCGCAATGAATAGGGA GGGTTATAGTTGAAGCAGCCA 50.0 47.6 57.9 58.0 991-1367 bp 1.0 76.6
psbE-petL_p3 CAACCCGCAATGAATAGGGA TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.9 58.0 993-1369 bp 1.0 76.6
psbE-petL_p4 ACTGACGAATAACCAACCCG GGGTTATAGTTGAAGCAGCCA 50.0 47.6 57.9 58.0 1004-1380 bp 1.0 76.3
psbE-petL_p5 ACTGACGAATAACCAACCCG TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.9 58.0 1006-1382 bp 1.0 76.3