Hotspot

petA-psbJ

LSC · 64367-65414 · 1048 bp

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Pi 0.00968
MarkerSeek score 55.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1048
variable_sites
20
pi
0.00968
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCTGTGATTTTGGCAC TATGGCTCCTATTCCGGGTT 50.0 50.0 58.0 57.9 1154-1170 bp 1.0 81.7
petA-psbJ_p2 GGCATCTGTGATTTTGGCAC TTATGGCTCCTATTCCGGGT 50.0 50.0 58.0 57.9 1155-1171 bp 1.0 81.7
petA-psbJ_p3 GAGTCCAAGGCCTTCTGTTC TATGGCTCCTATTCCGGGTT 55.0 50.0 58.2 57.9 1179-1195 bp 1.0 80.2
petA-psbJ_p4 GAGTCCAAGGCCTTCTGTTC TTATGGCTCCTATTCCGGGT 55.0 50.0 58.2 57.9 1180-1196 bp 1.0 80.2
petA-psbJ_p5 GTCCAAGGCCTTCTGTTCTT TATGGCTCCTATTCCGGGTT 50.0 50.0 57.7 57.9 1177-1193 bp 1.0 79.4