Hotspot

accD-psaI

LSC · 59904-60733 · 830 bp

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Pi 0.00955
MarkerSeek score 39.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
830
variable_sites
16
pi
0.00955
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCTACAACTGGCGGAGTTAC AAAGAAGCCATTGCGATTGC 55.0 45.0 58.0 58.0 1165-1166 bp 1.0 81.4
accD-psaI_p2 CCTACAACTGGCGGAGTTAC GGGCCACTACAGGAACAAAA 55.0 50.0 58.0 58.0 1134-1135 bp 1.0 81.4
accD-psaI_p3 CGTTCGCGGGTAAAAGAGTA AAAGAAGCCATTGCGATTGC 50.0 45.0 58.0 58.0 1089-1090 bp 1.0 81.3
accD-psaI_p4 CGTTCGCGGGTAAAAGAGTA GGGCCACTACAGGAACAAAA 50.0 50.0 58.0 58.0 1058-1059 bp 1.0 81.3
accD-psaI_p5 CCTACAACTGGCGGAGTTAC TAGGGCCACTACAGGAACAA 55.0 50.0 58.0 58.0 1136-1137 bp 1.0 81.2