Hotspot

rbcL-accD

LSC · 57601-58463 · 863 bp

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Pi 0.0111
MarkerSeek score 52.10
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
863
variable_sites
21
pi
0.0111
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 CCAGTGGATAAGCTAGATAAAGAGA ACAACATCGAATTGAACCAACA 40.0 36.4 57.5 57.1 906-927 bp 1.0 43.9
rbcL-accD_p2 CCAGTGGATAAGCTAGATAAAGAG ACAACATCGAATTGAACCAACA 41.7 36.4 56.2 57.1 906-927 bp 1.0 43.9
rbcL-accD_p3 CCAGTGGATAAGCTAGATAAAGAGA AGACAACATCGAATTGAACCAAC 40.0 39.1 57.5 57.9 908-929 bp 1.0 43.8
rbcL-accD_p4 CCAGTGGATAAGCTAGATAAAGAGA GACAACATCGAATTGAACCAACA 40.0 39.1 57.5 58.1 907-928 bp 1.0 43.8
rbcL-accD_p5 CCAGTGGATAAGCTAGATAAAGAGA AGACAACATCGAATTGAACCA 40.0 38.1 57.5 55.8 908-929 bp 1.0 43.8