Hotspot

trnE-UUC-trnT-GGU

LSC · 31877-32574 · 698 bp

Back to Phaius

Pi 0.00967
MarkerSeek score 60.30
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
698
variable_sites
13
pi
0.00967
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 751-870 bp 1.0 83.9
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.8 58.1 730-849 bp 1.0 83.5
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.8 58.1 727-846 bp 1.0 83.3
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 729-848 bp 1.0 83.1
trnE-UUC-trnT-GGU_p5 ATGACCAACTTCCTTTCGCA GAACCGATGACTTACGCCTT 45.0 50.0 57.7 58.0 801-920 bp 1.0 83.0