Hotspot

petN-psbM

LSC · 29399-30188 · 790 bp

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Pi 0.00615
MarkerSeek score 52.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
790
variable_sites
10
pi
0.00615
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GGGGAAGAAGTGGGCTTTAG CTTGCGCTTATTGCTACTGC 55.0 50.0 57.9 57.9 886-914 bp 1.0 81.0
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT CTTGCGCTTATTGCTACTGC 50.0 50.0 58.2 57.9 949-977 bp 1.0 80.6
petN-psbM_p3 GGGGAAGAAGTGGGCTTTAG ATTGCTACTGCGCTGTTCAT 55.0 45.0 57.9 58.3 877-905 bp 1.0 79.7
petN-psbM_p4 TATGGGGAAGAAGTGGGCTT CTTGCGCTTATTGCTACTGC 50.0 50.0 58.3 57.9 889-917 bp 1.0 79.4
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT ATTGCTACTGCGCTGTTCAT 50.0 45.0 58.2 58.3 940-968 bp 1.0 79.3