Hotspot

trnS-GCU-trnG-GCC

LSC · 8299-9334 · 1036 bp

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Pi 0.00921
MarkerSeek score 52.50
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1036
variable_sites
21
pi
0.00921
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1104-1228 bp 1.0 54.8
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1103-1227 bp 1.0 54.8
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1107-1231 bp 1.0 53.7
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1106-1230 bp 1.0 53.7
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1105-1229 bp 1.0 53.7