Hotspot

rps16-trnQ-UUG

LSC · 6070-6914 · 845 bp

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Pi 0.0117
MarkerSeek score 62.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
845
variable_sites
20
pi
0.0117
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 941-990 bp 1.0 80.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 918-967 bp 1.0 80.0
rps16-trnQ-UUG_p3 CCACAGCCAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 939-988 bp 1.0 79.8
rps16-trnQ-UUG_p4 ATCCACAGCCAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 945-994 bp 1.0 79.5
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 929-978 bp 1.0 79.4