Hotspot

rps15

SSC · 112645-112923 · 279 bp

Back to Phaeostigma

Pi 0.464

MarkerSeek score 76.80

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 124
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AATAATGGGAGCCGCTTGAG	TGAGGTAAATCAGCAGCGAC	50.0	50.0	58.0	58.0	1324 bp	0.5	59.7
rps15_p2	AGAATCAGAATGGTGCGCAT	TGAGGTAAATCAGCAGCGAC	45.0	50.0	58.0	58.0	1515 bp	0.5	59.5
rps15_p3	AATAATGGGAGCCGCTTGAG	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	893 bp	0.5	59.3
rps15_p4	AATAATGGGAGCCGCTTGAG	CCCTAATTGCTCTGGTGCAT	50.0	50.0	58.0	57.9	1088 bp	0.5	59.3
rps15_p5	AGAATCAGAATGGTGCGCAT	AACACCATGCATTGATGGGT	45.0	45.0	58.0	58.1	1084 bp	0.5	59.1