Hotspot

rpl32

SSC · 122687-122851 · 165 bp

Back to Phaeostigma

Pi 0.466

MarkerSeek score 76.30

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 165
variable_sites: 69
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	GGAAAAGAAGACTCCCCACC	TTTTCACGGATCTTTGACGT	55.0	40.0	57.8	55.6	605 bp	0.5	39.4
rpl32_p2	GGGAAAAGAAGACTCCCCAC	TTTTCACGGATCTTTGACGT	55.0	40.0	57.8	55.6	606 bp	0.5	39.4
rpl32_p3	GGAAAAGAAGACTCCCCACC	TCACGGATCTTTGACGTATCT	55.0	42.9	57.8	56.6	602 bp	0.5	39.0
rpl32_p4	GGGAAAAGAAGACTCCCCAC	TCACGGATCTTTGACGTATCT	55.0	42.9	57.8	56.6	603 bp	0.5	39.0
rpl32_p5	GGTGTCTGGTTTTAGGGCTT	TTTTCACGGATCTTTGACGT	50.0	40.0	57.7	55.6	1072 bp	0.5	38.7