Hotspot

ndhF

SSC · 123839-126070 · 2232 bp

Back to Phaeostigma

Pi 0.447

MarkerSeek score 76.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 745
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCTAAGATCCGGTGAATCG	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.2	57.9	2652 bp	0.5	52.4
ndhF_p2	AGCTAAGATCCGGTGAATCG	TCGTTGTGGTCGGACTCTAT	50.0	50.0	57.2	58.2	2831 bp	0.5	52.2
ndhF_p3	AGCTAAGATCCGGTGAATCG	ATCAATTCGGTCGTTGTGGT	50.0	45.0	57.2	57.8	2841 bp	0.5	52.1
ndhF_p4	CGAAAGAGCTAAGATCCGGT	CTAGCACTGGGTAGACCTCA	50.0	55.0	57.1	57.9	2658 bp	0.5	51.9
ndhF_p5	CGAAAGAGCTAAGATCCGGT	TCGTTGTGGTCGGACTCTAT	50.0	50.0	57.1	58.2	2837 bp	0.5	51.7