Hotspot

ndhD

SSC · 118898-120436 · 1539 bp

Back to Phaeostigma

Pi 0.464

MarkerSeek score 77.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1539
variable_sites: 535
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CATGTGTGCCACGTAAGGTA	GTAGTGATTCACGCGGTTCT	50.0	50.0	57.9	58.0	2171 bp	0.5	59.2
ndhD_p2	TGTGTGCCACGTAAGGTATG	GTAGTGATTCACGCGGTTCT	50.0	50.0	57.9	58.0	2169 bp	0.5	59.2
ndhD_p3	CATGTGTGCCACGTAAGGTA	CGCAAAGACCAAGCAGATTG	50.0	50.0	57.9	58.0	2151 bp	0.5	59.1
ndhD_p4	TGTGTGCCACGTAAGGTATG	CGCAAAGACCAAGCAGATTG	50.0	50.0	57.9	58.0	2149 bp	0.5	59.1
ndhD_p5	TGATACCTTGGGACGGATGT	GTAGTGATTCACGCGGTTCT	50.0	50.0	58.1	58.0	1908 bp	0.5	58.9