Hotspot

ndhA

SSC · 114204-116358 · 2155 bp

Back to Phaeostigma

Pi 0.482

MarkerSeek score 80.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2155
variable_sites: 904
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGAGTTGAAGCACCAAAGGG	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2507 bp	0.5	59.6
ndhA_p2	AGTTGAAGCACCAAAGGGAG	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2505 bp	0.5	59.6
ndhA_p3	GAGTTGAAGCACCAAAGGGA	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2506 bp	0.5	59.5
ndhA_p4	GTTGAAGCACCAAAGGGAGA	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2504 bp	0.5	59.5
ndhA_p5	CAACAGGCGCTAGAAGGAAT	TGACCAATATACCTTGCGGC	50.0	50.0	58.0	58.0	2666 bp	0.5	59.5