Hotspot

ccsA

SSC · 120639-121613 · 975 bp

Back to Phaeostigma

Pi 0.5

MarkerSeek score 79.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 385
pi: 0.5
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AGAACCGCGTGAATCACTAC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1249 bp	0.5	59.9
ccsA_p2	AGAACCGCGTGAATCACTAC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1248 bp	0.5	59.9
ccsA_p3	CAATCTGCTTGGTCTTTGCG	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1269 bp	0.5	59.7
ccsA_p4	CAATCTGCTTGGTCTTTGCG	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1268 bp	0.5	59.7
ccsA_p5	GTTTTTGATTCTGGACCGCG	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1498 bp	0.5	59.6