Hotspot

rpl32-ndhF

SSC · 122852-123838 · 987 bp

Back to Phaeostigma

Pi 0.446

MarkerSeek score 82.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 987
variable_sites: 403
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AATGCCGCCCAATATCCTTT	AGCAATAGCACCGGCAAATA	45.0	45.0	57.9	57.9	1790 bp	0.5	58.9
rpl32-ndhF_p2	AATGCCGCCCAATATCCTTT	ATTTAGCAATAGCACCGGCA	45.0	45.0	57.9	57.9	1794 bp	0.5	58.9
rpl32-ndhF_p3	AATGCCGCCCAATATCCTTT	TAGCAATAGCACCGGCAAAT	45.0	45.0	57.9	57.9	1791 bp	0.5	58.9
rpl32-ndhF_p4	AATGCCGCCCAATATCCTTT	AACAGGTCGTGTGAACCAAA	45.0	45.0	57.9	57.9	1583 bp	0.5	58.4
rpl32-ndhF_p5	AATGCCGCCCAATATCCTTT	ACAGGTCGTGTGAACCAAAA	45.0	45.0	57.9	57.9	1582 bp	0.5	58.4