Hotspot

trnL-TAG-rpl32

SSC · 121800-122686 · 887 bp

Back to Phaeostigma

Pi 0.422

MarkerSeek score 80.90

Primer available yes

Label trnL-TAG-rpl32

Feature Metadata

label_name: trnL-TAG-rpl32
feature_type: igs
region: SSC
length_bp: 887
variable_sites: 318
pi: 0.422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-TAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	AAAGGATATTGGGCGGCATT	50.0	45.0	58.0	57.9	1015 bp	0.5	59.2
trnL-TAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	AAAGGATATTGGGCGGCATT	50.0	45.0	58.0	57.9	1016 bp	0.5	59.2
trnL-TAG-rpl32_p3	TGATCCACAAAGAAGAGCCG	AAAGGATATTGGGCGGCATT	50.0	45.0	57.9	57.9	1640 bp	0.5	58.5
trnL-TAG-rpl32_p4	CTTAGTGGAAAATGGCCCGA	AAAGGATATTGGGCGGCATT	50.0	45.0	57.9	57.9	1378 bp	0.5	58.3
trnL-TAG-rpl32_p5	TCCTAAGAGCAGCGTGTCTA	AAAGGATATTGGGCGGCATT	50.0	45.0	57.9	57.9	1025 bp	0.5	58.3