Hotspot

rps15-ndhH

SSC · 112924-113014 · 91 bp

Back to Phaeostigma

Pi 0.541

MarkerSeek score 76.90

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 91
variable_sites: 46
pi: 0.541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	AATAATGGGAGCCGCTTGAG	TGAGGTAAATCAGCAGCGAC	50.0	50.0	58.0	58.0	1324 bp	0.5	59.7
rps15-ndhH_p2	AATAATGGGAGCCGCTTGAG	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	893 bp	0.5	59.3
rps15-ndhH_p3	AATAATGGGAGCCGCTTGAG	CCCTAATTGCTCTGGTGCAT	50.0	50.0	58.0	57.9	1088 bp	0.5	59.3
rps15-ndhH_p4	AATAATGGGAGCCGCTTGAG	AGGAGTCTGTGCCCCAATAT	50.0	50.0	58.0	58.1	1208 bp	0.5	59.0
rps15-ndhH_p5	AATAATGGGAGCCGCTTGAG	TTCCCTAATTGCTCTGGTGC	50.0	50.0	58.0	57.9	1090 bp	0.5	58.8