Hotspot

trnH-GTG-psbA

LSC · 75-465 · 391 bp

Back to Phaeostigma

Pi 0.0809
MarkerSeek score 88.90
Primer available yes
Label trnH-GTG-psbA

Feature Metadata

label_name
trnH-GTG-psbA
feature_type
igs
region
LSC
length_bp
391
variable_sites
28
pi
0.0809
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
88.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GTG-psbA_p1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 466-510 bp 1.0 84.9
trnH-GTG-psbA_p2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 480-524 bp 1.0 84.7
trnH-GTG-psbA_p3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 479-523 bp 1.0 84.7
trnH-GTG-psbA_p4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 481-525 bp 1.0 84.7
trnH-GTG-psbA_p5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 55.0 50.0 58.1 57.6 467-511 bp 1.0 84.4