Hotspot

EI075_pgp57-trnS(GCU)

Genome · 4874-6445 · 1572 bp

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Pi 0.171
MarkerSeek score 84.20
Primer available yes
Label EI075_pgp57-trnS(GCU)

Feature Metadata

label_name
EI075_pgp57-trnS(GCU)
feature_type
igs
region
Genome
length_bp
1572
variable_sites
255
pi
0.171
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp57-trnS(GCU)_p1 AGTCTGGTTTGTTTCCTTCT GTCTGAGTGGCCTAAAGAGC 40.0 55.0 54.4 58.0 2370 bp 0.5 31.0
EI075_pgp57-trnS(GCU)_p2 AGTCTGGTTTGTTTCCTTCT TCTGAGTGGCCTAAAGAGCT 40.0 50.0 54.4 58.1 2369 bp 0.5 30.6
EI075_pgp57-trnS(GCU)_p3 AGTCTGGTTTGTTTCCTTCT CCCTGTCATTCGTAGGTTCG 40.0 55.0 54.4 58.1 2431 bp 0.5 30.5
EI075_pgp57-trnS(GCU)_p4 AGTCTGGTTTGTTTCCTTCT TTTGGCCCTGTCATTCGTAG 40.0 50.0 54.4 57.9 2436 bp 0.5 30.2
EI075_pgp57-trnS(GCU)_p5 AGTCTGGTTTGTTTCCTTCT GGTGTAGCCAAGTGGTAAGG 40.0 55.0 54.4 57.9 2464 bp 0.5 30.2