Hotspot

EI075_pgp57

Genome · 3497-4873 · 1377 bp

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Pi 0.326
MarkerSeek score 82.50
Primer available yes
Label EI075_pgp57

Feature Metadata

label_name
EI075_pgp57
feature_type
gene
region
Genome
length_bp
1377
variable_sites
432
pi
0.326
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp57_p1 CGGTGGTCGTGATCAAGAAT AAAACTTCACCACCAGGACC 50.0 50.0 58.0 57.9 1905-2083 bp 1.0 91.4
EI075_pgp57_p2 CGGTGGTCGTGATCAAGAAT AGCAATCCATCCATCACCAC 50.0 50.0 58.0 57.9 2263 bp 0.5 59.4
EI075_pgp57_p3 TGATGGGTGTAGCTGGTGTA AGCAATCCATCCATCACCAC 50.0 50.0 58.1 57.9 2745 bp 0.5 59.1
EI075_pgp57_p4 CGGTGGTCGTGATCAAGAAT CACCAAATCCTAGAACGGCA 50.0 50.0 58.0 57.9 1973 bp 0.5 59.1
EI075_pgp57_p5 TGATGGGTGTAGCTGGTGTA AAAACTTCACCACCAGGACC 50.0 50.0 58.1 57.9 2387 bp 0.5 59.0