Hotspot

EI075_pgp40

Genome · 25133-26083 · 951 bp

Back to Phacus

Pi 0.256
MarkerSeek score 83.00
Primer available yes
Label EI075_pgp40

Feature Metadata

label_name
EI075_pgp40
feature_type
gene
region
Genome
length_bp
951
variable_sites
226
pi
0.256
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp40_p1 GCGATATGGCCAAGTGGTAA TTCACCATTGGGCAACTCAA 50.0 45.0 58.0 57.9 1623 bp 0.5 59.1
EI075_pgp40_p2 CGAATCTGGGTGTCGTCTTT TTCACCATTGGGCAACTCAA 50.0 45.0 57.9 57.9 1571 bp 0.5 58.7
EI075_pgp40_p3 GCGATATGGCCAAGTGGTAA CAAGTGCCCCAGACCAATTA 50.0 50.0 58.0 57.8 1588 bp 0.5 58.0
EI075_pgp40_p4 CGAATCTGGGTGTCGTCTTT CAAGTGCCCCAGACCAATTA 50.0 50.0 57.9 57.8 1536 bp 0.5 57.6
EI075_pgp40_p5 GCGATATGGCCAAGTGGTAA AGCCAAACCTGCAGTTGTAT 50.0 45.0 58.0 57.7 1668 bp 0.5 57.4