Hotspot

EI075_pgp37

Genome · 27338-27895 · 558 bp

Back to Phacus

Pi 0.242
MarkerSeek score 80.00
Primer available yes
Label EI075_pgp37

Feature Metadata

label_name
EI075_pgp37
feature_type
gene
region
Genome
length_bp
558
variable_sites
132
pi
0.242
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp37_p1 GGTCAAGGTACTGCATCTGG CTGGAGCTGGCGATTCTATC 55.0 55.0 58.0 57.9 1313 bp 0.5 59.1
EI075_pgp37_p2 GGTCAAGGTACTGCATCTGG GGAGCTGGCGATTCTATCAG 55.0 55.0 58.0 57.9 1311 bp 0.5 59.1
EI075_pgp37_p3 GGCTTAGGTGCTATTGGTCC CTGGAGCTGGCGATTCTATC 55.0 55.0 58.0 57.9 1340 bp 0.5 59.1
EI075_pgp37_p4 GGCTTAGGTGCTATTGGTCC GGAGCTGGCGATTCTATCAG 55.0 55.0 58.0 57.9 1338 bp 0.5 59.1
EI075_pgp37_p5 GGTCAAGGTACTGCATCTGG TTCGCCTGCCATAACCTTTT 55.0 45.0 58.0 58.1 1063 bp 0.5 59.1