Hotspot

EI075_pgp33

Genome · 30771-31350 · 580 bp

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Pi 0.392
MarkerSeek score 82.00
Primer available yes
Label EI075_pgp33

Feature Metadata

label_name
EI075_pgp33
feature_type
gene
region
Genome
length_bp
580
variable_sites
220
pi
0.392
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
EI075_pgp33_p1 GGATGTAGCGCAGTTTGGTA TTTTGGAAGAAGCTGGTGCA 50.0 45.0 58.0 58.2 1402 bp 0.5 58.0
EI075_pgp33_p2 GGATGTAGCGCAGTTTGGTA TTACAAGTGCAGTTTGGGCA 50.0 45.0 58.0 58.2 1183 bp 0.5 58.0
EI075_pgp33_p3 ACGTTTGTTACCGAGGGTTC TTTTGGAAGAAGCTGGTGCA 50.0 45.0 58.1 58.2 1277 bp 0.5 57.2
EI075_pgp33_p4 CGTTTGTTACCGAGGGTTCA TTTTGGAAGAAGCTGGTGCA 50.0 45.0 58.1 58.2 1276 bp 0.5 57.1
EI075_pgp33_p5 ACGTTTGTTACCGAGGGTTC TTACAAGTGCAGTTTGGGCA 50.0 45.0 58.1 58.2 1058 bp 0.5 57.1