Hotspot

rrn5-rrn4.5

IRa · 116138-116345 · 208 bp

Back to Phacellaria

Pi 0.0128

MarkerSeek score 61.70

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 208
variable_sites: 3
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	AGTATCGTCACCGCAGTAGA	GAGACGAGCCGTTTCTCATT	50.0	50.0	58.0	58.0	377-380 bp	1.0	81.0
rrn5-rrn4.5_p2	TATCGTCACCGCAGTAGAGT	GAGACGAGCCGTTTCTCATT	50.0	50.0	58.0	58.0	375-378 bp	1.0	81.0
rrn5-rrn4.5_p3	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	322-325 bp	1.0	80.8
rrn5-rrn4.5_p4	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	320-323 bp	1.0	80.8
rrn5-rrn4.5_p5	TCCCCTACAGTATCGTCACC	GAGACGAGCCGTTTCTCATT	55.0	50.0	57.9	58.0	385-388 bp	1.0	80.7