Hotspot

psaC-rps15

SSC · 107741-108707 · 967 bp

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Pi 0.0368

MarkerSeek score 73.30

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 967
variable_sites: 48
pi: 0.0368
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	CGTGGAGCAGAAGCAATTTG	TTGGGAAAACGTCAACGACT	50.0	45.0	58.0	58.0	1157-1161 bp	1.0	86.4
psaC-rps15_p2	GCTTTACATCCCTCCCAAGG	TTGGGAAAACGTCAACGACT	55.0	45.0	57.9	58.0	1133-1137 bp	1.0	86.3
psaC-rps15_p3	TCGTGGAGCAGAAGCAATTT	TTGGGAAAACGTCAACGACT	45.0	45.0	58.1	58.0	1158-1162 bp	1.0	85.8
psaC-rps15_p4	TTCGTGGAGCAGAAGCAATT	TTGGGAAAACGTCAACGACT	45.0	45.0	58.1	58.0	1159-1163 bp	1.0	85.8
psaC-rps15_p5	GACCCATACTGCGAGTTGTT	TTGGGAAAACGTCAACGACT	50.0	45.0	57.9	58.0	1261-1265 bp	1.0	85.3