Hotspot

rpl32-trnL(UAG)

SSC · 104467-105033 · 567 bp

Back to Phacellaria

Pi 0.0282

MarkerSeek score 66.50

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 567
variable_sites: 22
pi: 0.0282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	GAGCTACTGAGGAACAACGG	ACCTATTTCACCATAGCGGC	55.0	50.0	58.0	57.7	1123-1134 bp	1.0	81.9
rpl32-trnL(UAG)_p2	AGAATCGCGCAGAACAGAAT	ACCTATTTCACCATAGCGGC	45.0	50.0	58.0	57.7	1324-1330 bp	1.0	81.4
rpl32-trnL(UAG)_p3	GAGCTACTGAGGAACAACGG	GTGCGGTTATTGCGCTTAAG	55.0	50.0	58.0	58.2	1614-1615 bp	0.666667	65.7
rpl32-trnL(UAG)_p4	AGAATCGCGCAGAACAGAAT	GTGCGGTTATTGCGCTTAAG	45.0	50.0	58.0	58.2	1810-1811 bp	0.666667	65.6
rpl32-trnL(UAG)_p5	TCGTATAGTGGCTCCGTTCT	GTGCGGTTATTGCGCTTAAG	50.0	50.0	58.0	58.2	1510-1511 bp	0.666667	65.4