Hotspot

trnF(GAA)-tRNA-Met(CAU)

LSC · 47347-48371 · 1025 bp

Back to Phacellaria

Pi 0.0135

MarkerSeek score 64.10

Primer available yes

Label trnF(GAA)-tRNA-Met(CAU)

Feature Metadata

label_name: trnF(GAA)-tRNA-Met(CAU)
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 19
pi: 0.0135
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(GAA)-tRNA-Met(CAU)_p1	TGATGCTTCAGGAATGGTCG	CGCCGTATGAAAGCAGTACT	50.0	50.0	58.0	58.1	1092-1156 bp	1.0	84.5
trnF(GAA)-tRNA-Met(CAU)_p2	CCTCGTGTCACCAGTTCAAA	CGCCGTATGAAAGCAGTACT	50.0	50.0	58.1	58.1	1037-1101 bp	1.0	84.4
trnF(GAA)-tRNA-Met(CAU)_p3	AATGGTCGGGATAGCTCAGT	CGCCGTATGAAAGCAGTACT	50.0	50.0	58.2	58.1	1080-1144 bp	1.0	83.1
trnF(GAA)-tRNA-Met(CAU)_p4	ATGGTCGGGATAGCTCAGTT	CGCCGTATGAAAGCAGTACT	50.0	50.0	58.2	58.1	1079-1143 bp	1.0	83.1
trnF(GAA)-tRNA-Met(CAU)_p5	GAATGGTCGGGATAGCTCAG	CGCCGTATGAAAGCAGTACT	55.0	50.0	57.6	58.1	1081-1145 bp	1.0	81.2